tgfβ receptor i  (Bioss)

Journal: Frontiers in Veterinary Science

Article Title: All-trans retinoic acid modulates proliferation and apoptosis of secondary hair follicle–dermal papilla cells in cashmere goats via the TGF- β 2/Smad2/3 pathway

doi: 10.3389/fvets.2026.1765302

Figure Lengend Snippet: ATRA alters the transcriptome of cashmere goat SHF-DPCs. (a) Principal component analysis (PCA) of transcriptome sequencing data from the control and ATRA-treated (10 −4 M, 24 h) groups. (b) Volcano plot showing differentially expressed genes (DEGs) between the control and ATRA-treated groups ( p < 0.05 and |log₂ fold change (FC)| > 1). (c) Relative mRNA expression of transforming growth factor-β2 (TGF- β 2) in the control and ATRA-treated groups (qRT-PCR). (d) Correlation between RNA sequencing and qRT-PCR data for selected DEGs based on log₂FC values; (e) Gene Ontology enrichment analysis of DEGs. (f) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment bubble plot of DEGs. (g) Western blot analysis of TGF- β 2 expression in the control and ATRA-treated groups with corresponding quantitative analysis. (h) Western blot analysis of Smad2/3 phosphorylation levels in the control and ATRA-treated groups with corresponding quantitative analysis. Data are presented as the mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: SHF-DPCs were seeded into 96-well plates at a density of 5 × 10 3 cells/well in 100 μL of medium and cultured at 37 °C with 5% CO2 for 24 h. After removal of the complete culture medium, cells were washed with PBS and treated with ATRA (HY-14649, MedChemExpress, Monmouth Junction, NJ, United States) at different concentrations (10 −8 , 10 −7 , 10 −6 , 10 −5 , 10 −4 , 10 −3 M) for 24, 48 or 72 h. For mechanistic validation experiments, SHF-DPCs were treated with ATRA (10 −4 M) and/or LY2109761 (2 μM), a TGF- β type I/II receptor inhibitor (S2704; Selleck Chemicals, Houston, TX, United States), for 24 h. ATRA and LY2109761 were prepared in complete culture medium, and the control group received an equal volume of culture medium.

Techniques: Sequencing, Control, Expressing, Quantitative RT-PCR, RNA Sequencing, Western Blot, Phospho-proteomics

Journal: Frontiers in Veterinary Science

Article Title: All-trans retinoic acid modulates proliferation and apoptosis of secondary hair follicle–dermal papilla cells in cashmere goats via the TGF- β 2/Smad2/3 pathway

doi: 10.3389/fvets.2026.1765302

Figure Lengend Snippet: ATRA regulates proliferation and apoptosis in cashmere goat SHF-DPCs via the TGF- β 2/Smad2/3 pathway. (a) Representative images of EdU staining of SHF-DPCs treated with ATRA (10 −4 M) LY2109761 (2 μM), or ATRA + LY2109761 (scale bar = 200 μm). (b) Representative images of TUNEL staining of SHF-DPCs treated with ATRA, LY2109761, or ATRA + LY2109761 (scale bar = 200 μm). (c) CCK-8 assay to assess cell viability of SHF-DPCs treated with ATRA, LY2109761, or ATRA + LY2109761. (d) Western blot analysis of Bcl-2 and Bax expression in SHF-DPCs treated with ATRA, LY2109761, or ATRA + LY2109761 with corresponding quantitative analysis. (e) Western blot analysis of Smad2/3 phosphorylation levels in SHF-DPCs treated with ATRA, LY2109761, or ATRA + LY2109761 with corresponding quantitative analysis. Data are presented as the mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.

Article Snippet: SHF-DPCs were seeded into 96-well plates at a density of 5 × 10 3 cells/well in 100 μL of medium and cultured at 37 °C with 5% CO2 for 24 h. After removal of the complete culture medium, cells were washed with PBS and treated with ATRA (HY-14649, MedChemExpress, Monmouth Junction, NJ, United States) at different concentrations (10 −8 , 10 −7 , 10 −6 , 10 −5 , 10 −4 , 10 −3 M) for 24, 48 or 72 h. For mechanistic validation experiments, SHF-DPCs were treated with ATRA (10 −4 M) and/or LY2109761 (2 μM), a TGF- β type I/II receptor inhibitor (S2704; Selleck Chemicals, Houston, TX, United States), for 24 h. ATRA and LY2109761 were prepared in complete culture medium, and the control group received an equal volume of culture medium.

Techniques: Staining, TUNEL Assay, CCK-8 Assay, Western Blot, Expressing, Phospho-proteomics